Department of Genetics and Animal Breeding

Faculty of Veterinary Medicine and Animal Sciences
Poznan University of Life Sciences

Animal Genomics and Genetic Diagnostics Group

Group members:

Our main research interests:

  • Cytogenetic and molecular background of hereditary diseases and congenital malformations, in particular disorders of sex development
  • Genomics and epigenomics of fatness/obesity traits
  • Nutrigenomics
  • Architecture of interphase nuclei
  • CRISPR/Cas9 genome editing of porcine mesenchymal stem cells (MSC)
  • Genomics and epigenomics of cancers
  • Bioinformatics of DNA sequences

Applied research techniques:

  • In vitro cell cultures (lymphocytes, fibroblasts, MSC, cancer cell lines) 
  • Classical (chromosome staining, karyotyping) and molecular cytogenetics (fluorescence in situ hybridization – FISH, 3D-FISH, RNA-FISH, immuno-FISH)
  • Immunofluorescent staining (IF) 
  • Sanger sequencing 
  • NGS sequencing (MiSeq)
  • Real-time PCR
  • Droplet digital PCR (ddPCR)
  • DNA methylation analysis by pyrosequencing
  • Chromatin immunoprecipitation (ChIP)

Selected Publications

2022

Flisikowski K, Perleberg C, Niu G, Winogrodzki T, Bak A, Liang W, Grodziecki A, Zhang Y, Pausch H, Flisikowska T, Klinger B, Perkowska A, Kind A, Switonski M, Janssen KP, Saur D, Schnieke A. (2022). Wildtype APC influences the severity of familial adenomatous polyposis. Cellular and Molecular Gastroenterology and Hepatology 13(2): 669-671. 

Krzeminska P., Nowacka-Woszuk J., Switonski M. (2022). Copy number variation of the SRY gene showed an association with disorders of sex development in Yorkshire Terrier dogs. Animal Genetics 53(1): 152-155. 

Krzeminska P, Nowak T, Switonski M. (2022 – online early). Isolated cryptorchidism in dogs is not associated with polymorphisms of the INSL3 and AR candidate genes. Animal Genetics,  doi: 10.1111/age.13175. 

 

2021

Naillat F., Saadeh H., Nowacka-Woszuk J., Gahurova L., Santos F., Tomizawa S., Kelsey G. (2021), Oxygen concentration affects de novo DNA methylation and transcription in in vitro cultured oocytes. Clinical Epigenetics, 13, 132.

Nowacka-Woszuk J., Mackowski M., Mantaj W., Stefaniuk-Szmukier M., Cieslak J. (2021). Equine STX17 intronic triplication confirmed by droplet digital PCR analysis of its breakpoints. Animal Genetics, 52: 567-56.

Nowacka-Woszuk J., Mackowski M., Stefaniuk-Szmukier M., Cieslak J. (2021). The equine graying with age mutation of the STX17 gene: A copy number study using droplet digital PCR reveals a new pattern. Animal Genetics 11, 223-227.

Nowacka-Woszuk J. (2021). The genetic background of hernia in pigs: A review. Livestock Science 244: 104317. 

Shaffer L.G., Hopp B., Switonski M. Zahland A., Blake C. Ballif B.C. (2021).  Identification of aneuploidy in dogs screened by a SNP microarray. Human Genetics 140: 1619-1624. 

Stachecka J., Kolodziejski P.A., Noak M., Szczerbal I. (2021). Alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells. Scientific Reports 11, 1325.

Szczerbal I., Switonski M. (2021). Clinical Cytogenetics of the Dog: A Review. Animals 11, 947. 

Szczerbal I., Nizanski W., Dzimira S., Nowacka-Woszuk J., Stachecka J. Biezynski J., Ligocka Z., Jagodka D., Fabian-Kurzok H., Switonski M. (2021). Chromosome abnormalities in dogs with disorders of sex development (DSD). Animal Reproduction Science 230, 106771. 

2020

Original papers

Szczerbal I., Nowacka-Woszuk J., Kopp-Kuhlman C., Mackowski M., Switonski M. (2020). Application of droplet digital PCR in diagnosing of X monosomy in mares. Equine Veterinary Journal 52: 627-631.

Sikorska A, Stachowiak M, Flisikowska T, Stachecka J, Flisikowski K, Switonski M. (2020). Polymorphisms of CSF1R and WISP1 genes are associated with severity of familial adenomatous polyposis in APC1311 pigsGene 759:144988.

Stachecka J., Lemanska W., Noak M., Szczerbal I. (2020). Expression of key genes involved in DNA methylation during in vitro differentiation of porcine mesenchymal stem cells (MSCs) into adipocytes. Biochemical and Biophysical Research Communications 522: 811-818.

Krzeminska P, Stachowiak M, Skrzypski M, Nowak T, Maslak A, Switonski M. (2020 – online early). Altered expression of CYP17A1 and CYP19A1 in undescended testes of dogs with unilateral cryptorchidismAnimal Genetics.  51: 763-771.

Nowacka-Woszuk J., Krzeminska P., Nowak T, Gogulski M, Switonski M, Stachowiak M (2020). Analysis of transcript and methylation levels of INSL3 and RXFP2 in undescended and descended dog testes suggested promising biomarkers associated with cryptorchidism. Theriogenology 157:483-489

Antkowiak M., Nowacka-Woszuk J., Szczerbal I., Switonski M., Szydlowski M. (2020). AMY2B gene copy-number variation studied by droplet digital PCR (ddPCR) in three canids: red fox, arctic fox, and Chinese raccoon dogFolia Biologica (Kraków) 68: 51-55.

Review articles

Nowacka-Woszuk J. (2020), Nutrigenomics in livestock-recent advances. Journal of Applied Genetics 61 (1): 93-103.

Switonski M (2020).  Impact of gene therapy for canine monogenic diseases on the progress of preclinical studies, Journal of Applied Genetics, 61:179-186.

Szczerbal I, Switonski M. (2020). Genetic disorders of sex development in cats: An update, Animal Reproduction Science 216: 106353.

  • Present projects

National Science Centre in Poland (NCN) - OPUS: The application of transcriptome profiling to identify genes associated with umbilical hernia in pigs, principal investigator: Dr. habil. Joanna Nowacka – Woszuk, 2020-2023.

National Science Centre in Poland (NCN) - OPUS: The application of CRISPR-Cas9 technology to elucidate whether changes in nuclear architecture during adipogenesis are cause or a consequence, project no. 2018/29/B/NZ2/00956, principal investigator: Prof. dr. habil. Izabela Szczerbal, 2019-2022.

National Science Centre in Poland (NCN) - OPUS: Identification of genetic and epigenetic markers associated with cryptorchidism in dogs, project no. 2018/29/B/NZ9/01065, principal investigator: Prof. dr. habil. Marek Świtoński, 2019-2022.

National Science Centre in Poland (NCN) - OPUS: A comprehensive characteristics of candidate genomic regions for canine monogenic disorders of sex development, analyzed with the use of next generation sequencing (NGS) and droplet digital PCR (ddPCR), project no. 2016/23/B/NZ9/03424, principal investigator: Prof. dr. habil. Marek Świtoński, 2017-2021. 

National Science Centre in Poland (NCN) - OPUS: Identification of obesity risk variants in the dog genome using whole-genome sequencing  , project no. 2016/23/B/NZ2/01762, principal investigator: Prof. dr. habil. Maciej Szydłowski, 2017-2021.

National Science Centre in Poland (NCN) - SONATA: Identifying molecular events for transformation of early colorectal polyps. Studies with APC1311-targeted pigs, project no. 2016/21/D/NZ2/03830, principal investigator: Dr. habil. Monika Dragan, 2017-2021.

  • List of the main courses led by the members of the group:

General and Veterinary Genetics, Veterinary Medicine, Person in charge: Prof. Marek Świtoński

Genetics , Biotechnology (First-Cycle Studies), Person in charge: Prof. Marek Świtoński (the course led jointly with Department of Genetics and Plant Breeding, Faculty of Agronomy, Horticulture and Bioengineering)

Genetics, Biology (First-Cycle Studies), Person in charge: Prof. Marek Świtoński

Cytogenetics and Chromosome Engineering, Biotechnology (First-Cycle Studies), Person in charge: Prof. Marek Świtoński (the course led jointly with Department of Genetics and Plant Breeding, Faculty of Agronomy, Horticulture and Bioengineering)

Human Genetics, Biology (Second-Cycle Studies), Person in charge: Prof. Marek Świtoński

Diagnostics of Genetic Diseases in Animals, Veterinary Medicine, Person in charge: Prof. Marek Świtoński

Bioinformatics, Biology (Second-Cycle Studies) and Biotechnology (Second-Cycle Studies), Person in charge: Prof. Maciej Szydłowski

Molecular genetics and principles of biotechnology, Biology (First-Cycle Studies), Person in charge: Dr. Habil. Monika Dragan

Genomics and transgenic animals, Biotechnology (Second-Cycle Studies), Person in charge: Dr. Habil. Joanna-Nowacka-Woszuk

Nutrigenomic and molecular diagnostics – Animal nutrition (Second-Cycle Studies, dual study), Person in charge: Dr. Habil. Joanna-Nowacka-Woszuk

Diagnostyka cyto-immunogenetyczna zwierząt – kierunek Biologia (II°) – kierownik –  prof. dr hab. Izabela Szczerbal

Genetic diagnostics in animal breeding – kierunek Zootechnika – kierownik –  prof. dr hab. Izabela Szczerbal

Molecular genetics and epigenetics – Biotechnology (First-Cycle Studies) - Person in charge: Prof. Izabela Szczerbal (the course led jointly with Department of Biochemistry and Biotechnology, Faculty of Agronomy, Horticulture and Bioengineering)

Neurogenetics – Neurobiology (Second-Cycle Studies) – Person in charge: Prof. Izabela Szczerbal and Dr. Habil. Monika Dragan (the course led jointly with AMU and PUMS)

Genetic diseases and their diagnostics i ich diagnostyka–   kierunek – Biotechnologia (II°), kierownik prof. Marek Świtoński

  • Courses in English:

Recent advances in Animal Biotechnology – MSc in Biotechnology (Second-Cycle Studies); Biotechnology (Second-Cycle Studies) – Person in charge: Prof. Marek Świtoński

Animal Genomics – kierunek Animal Production Management (II°) –  kierownik prof. Marek Świtoński

Genetic diagnostics in Animal Breeding – Animal Production Management (Second-Cycle Studies) – Person in charge: Prof. Marek Świtoński

Genetic Diseases of Domestic Animals – Biotechnology (Second-Cycle Studies) – Person in charge: Prof. Marek Świtoński

Cytogenetics and Chromosome Engineering – MSc in Biotechnology (Second-Cycle Studies), Person in charge: Prof. Marek Świtoński (the course led jointly with Department of Biochemistry and Biotechnology, Faculty of Agronomy, Horticulture and Bioengineering)

 

Department of Genetics and Animal Breeding

Faculty of Veterinary Medicine and Animal Sciences
Poznan University of Life Sciences